ICC/IF
Immunofluorescence analysis of C2C12 cells using ATP1A1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-ATP1A1 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATP1A1 |
|---|---|
| Gene Synonyms | Atpa-1 CMT2DD HOMGSMR2 Nkaa1b |
| Gene Full Name | ATPase Na+/K+ transporting subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Alternative Names | (Na+ + K+)-ATPase (Na+ and K+) ATPase alpha catalytic subunit precursor (Na+ K+)-ATPase alpha-subunit ATP1B ATPase ATPase Na+/K+ transporting alpha 1 polypeptide ATPase Na+K+ transporting alpha 1 G00-119-711 MGC1798 Na(+)/K(+) ATPase alpha-1 subunit Na+ K+ -ATPase alpha subunit Na+ /K+ ATPase alpha 1 subunit Na+ K+ ATPase alpha subunit Na+ K+-ATPase beta-subunit precursor Na+/K+ ATPase 1 Na+/K+ ATPase Alpha Na+/K+ transporting alpha 1 polypeptide Na K-ATPase alpha-A catalytic polypeptide Na K-ATPase alpha-1 subunit Na K-ATPase catalytic subunit alpha-A protein Na K-ATPase alpha-subunit RP4-655J12.1 Sodium Potassium ATPase sodium pump 1 sodium pump subunit alpha-1 sodium-potassium ATPase catalytic subunit alpha-1 sodium-potassium-ATPase alpha 1 polypeptide sodium/potassium-transporting ATPase alpha-1 chain |
| Cellular Localization | Cell membrane. Melanosome. |
| SwissProt ID | P05023 |
|---|
ICC/IF
Immunofluorescence analysis of C2C12 cells using ATP1A1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
