ICC/IF
Immunofluorescence analysis of C2C12 cells using GKAP antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-GKAP antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DLGAP1 |
|---|---|
| Gene Synonyms | 4933422O14Rik 6430596N04Rik 9630002F18 C8orf68 D17Bwg0511e DAP-1 DAP-1-ALPHA DAP-1-BETA DAP-2 DAP1 DAP2 DLGAP1A DLGAP1B ERICH1-AS1 GKAP GKAP/SAPAP GKAP/SAPAP1 GKPA/SAPAP mKIAA4162 rGKAP SAPAP1 SAPAP2 |
| Gene Full Name | DLG associated protein 1 |
| Gene Summary | Predicted to enable molecular adaptor activity. Predicted to be a structural constituent of postsynaptic density. Predicted to be involved in several processes, including aggresome assembly; regulation of postsynaptic neurotransmitter receptor activity; and regulation of proteasomal protein catabolic process. Predicted to be located in plasma membrane. Predicted to be part of postsynaptic density. Predicted to be active in glutamatergic synapse and postsynaptic density, intracellular component. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | DAP-1 beta discs large homolog associated protein 1 discs large homolog associated protein 2 discs large-associated protein 2 discs large homolog-associated protein 1 discs large homolog-associated protein 2 ERICH1 antisense gene protein 1 ERICH1 antisense RNA 1 (non-protein coding) guanylate kinase-associated protein postsynaptic density 95 PSD-95 binding protein PSD-95/SAP90 binding protein 1 PSD-95/SAP90-associated protein-2 PSD-95/SAP90-binding protein 1 PSD-95/SAP90-binding protein 2 putative DAP-2 like protein SAP90/PSD-95 associated protein 1 SAP90/PSD-95 associated protein 2 SAP90/PSD-95-associated protein 1 SAP90/PSD-95-associated protein 2 SAPAP-1 SAPAP-2 |
| Molecular Weight(MW) | 109KD |
| Cellular Localization | Cell junction, Cell membrane, Membrane, Synapse |
| SwissProt ID | O14490 |
|---|
ICC/IF
Immunofluorescence analysis of C2C12 cells using GKAP antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
