WB
Western blot analysis using ADARB1 antibody against Hela(1),A549(2),Huvec(3),Cos-7(4)cell. 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | ADARB1 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ADAR |
|---|---|
| Gene Synonyms | ADAR1 Adar1p110 Adar1p150 AGS6 DRADA DSH DSRAD G1P1 IFI-4 IFI4 K88DSRBP mZaADAR P136 |
| Gene Full Name | adenosine deaminase RNA specific |
| Gene Summary | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Alternative Names | 136 kDa double-stranded RNA-binding protein ADA Adar ADAR1 ADAR1La ADAR1Lb ADAR1Sa Adenosine Deaminase adenosine deaminase acting on RNA 1-A DRADA dsRNA adenosine deaminase dsRNA adeonosine deaminase IFI-4 interferon-induced protein 4 interferon-inducible protein 4 RNA adenosine deaminase 1 RNA-specific adenosine deaminase p110 form RNA-specific adenosine deaminase p150 form |
| Molecular Weight(MW) | 136kDa（observed：110kDa) |
| Cellular Localization | Cytoplasm;Nucleus;Nucleus, nucleolus |
| SwissProt ID | P55265 |
|---|
WB
Western blot analysis using ADARB1 antibody against Hela(1),A549(2),Huvec(3),Cos-7(4)cell. 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
