WB
WB analysis using ADAR1 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ADAR1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
IHC staining of human colon cancer using ADAR1 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using ADAR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of mouse brain using ADAR1 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using ADAR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of HeLa using ADAR1 Polyclonal antibody. Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADAR1 antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).IP
IP experiment of HepG2 using ADAR1 Polyclonal antibody. IP result of anti-ADAR1 (IP: 4ug; Detection:1:800) with HepG2 cells lysate 1320 ug.| Product Name | ADAR1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ADAR1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAR |
|---|---|
| Gene Synonyms | DSH AGS6 G1P1 IFI4 P136 ADAR1 DRADA DSRAD IFI-4 K88DSRBP |
| Gene Full Name | adenosine deaminase RNA specific |
| Gene Summary | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Alternative Names | ADAR 136 kDa double-stranded RNA-binding protein adenosine deaminase RNA specific Double-stranded RNA-specific adenosine deaminase DRADA |
| Molecular Weight(MW) | 136 kDa(Observed band size:110 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
WB analysis using ADAR1 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with ADAR1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human colon cancer using ADAR1 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using ADAR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of mouse brain using ADAR1 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using ADAR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of HeLa using ADAR1 Polyclonal antibody. Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADAR1 antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IP
IP experiment of HepG2 using ADAR1 Polyclonal antibody. IP result of anti-ADAR1 (IP: 4ug; Detection:1:800) with HepG2 cells lysate 1320 ug.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
