ICC/IF
Immunofluorescence analysis of HUVEC cells using PPARGC1B antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-PPARGC1B antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPARGC1B |
|---|---|
| Gene Synonyms | 4631412G21Rik A830037N07Rik ERRL1 Gm11133 LEM6 LRPGC1 PERC PGC-1 PGC-1(alpha) PGC-1(beta) PGC-1alpha PGC-1beta PGC-1beta/ERRL1 PGC-1v PGC1 PGC1A PGC1B PGC1beta Pgco1 PGCvf PGCvf-1 PGCvf1 PPARGC-1-alpha PPARGC-1-beta PPARGC1 |
| Gene Full Name | PPARG coactivator 1 beta |
| Gene Summary | The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| Alternative Names | ERR ligand 1 L-PGC-1alpha ligand effect modulator-6 PERC PERC-s lacks one estrogen receptor interaction domain peroxisome proliferative activated receptor gamma coactivator 1 peroxisome proliferative activated receptor gamma coactivator 1 alpha peroxisome proliferative activated receptor gamma coactivator 1 beta peroxisome proliferator-activated receptor gamma coactivator 1beta-2a peroxisome proliferator-activated receptor gamma coactivator 1 beta PGC-1 pgc-1 alpha pgc-1 beta PGC-1-alpha PGC-1-beta PGC-1-related estrogen receptor alpha coactivator PGC-1alpha PGC1beta-1a PGC1beta-1b PGC1beta-2a PGC1beta-2b PPAR gamma coactivator 1alpha variant form-1 PPAR gamma coactivator variant form PPAR gamma coactivator-1beta protein PPAR-gamma coactivator 1-alpha PPAR-gamma coactivator 1-beta PPARAGCIalpha PPARAGCIbeta PPARgamma coactivator 1 beta PPARgamma coactivator 1alpha PPARGC-1-alpha PPARGC-1-beta Ppargc1a-vf1 transcriptional coactivator transcriptional coactivator PGC-1 |
| Molecular Weight(MW) | 113kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q86YN6 |
|---|
ICC/IF
Immunofluorescence analysis of HUVEC cells using PPARGC1B antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
