Application
Anti-FH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FH monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FHmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-FH mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.Application
HEK293T cells transfected with either Fumarate hydratase overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody , and then analyzed by flow cytometry.| Product Name | Fumarate hydratase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9G4] to FHAlso acts as a tumor suppressor. |
| Immunogen | Protein expressed in 293T cell transfected with human FH expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9G4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanDogRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FH MCUL1 MCL LRCC HLRCC |
|---|---|
| Target | FH |
| Tissue Specificity | is specific for human FH. |
Application
Anti-FH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FH monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FHmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-FH mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
Application
HEK293T cells transfected with either Fumarate hydratase overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody , and then analyzed by flow cytometry.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM253707