Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the detected the FH protein (arrow).| Product Name | FH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This FH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-135 amino acids from the N-terminal region of human FH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FH |
|---|---|
| Alternative Names | Fumarate hydratase mitochondrial Fumarase FH |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the detected the FH protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM278844