All products Antibodies 6X His tag antibody

6X His tag antibody

Catalog Number：OM237660

  • 6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.

  • Sample A: 10 µg of non-transformed E. coli lysate B: 10 µg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.

  • Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Profile

Product Name 6X His tag antibody
Antibody Type Tags Antibodies
Product description 
Rabbit Polyclonal antibody to 6X His tag

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAIHC-WIPWB
*Optimal dilutions/concentrations should be determined by the researcher.:
WB:1:500~1:2000
IP:1:100~1:500
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
6X His tag antibody
His antibody
6x His antibody
6x his tag antibody
His Tag antibody
6xHis Tag antibody
6 x His antibody

Application

  • Application

    6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.

  • Application

    Sample A: 10 µg of non-transformed E. coli lysate B: 10 µg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.

  • Application

    Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control His-tagged recombinant protein
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.:
WB:1:500~1:2000
IP:1:100~1:500

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM237660
  • Price: $336.00
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  • Lead time: 4~6 Week

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