6X His tag antibody

Product Profile

Product Name	6X His tag antibody
Antibody Type	Tags Antibodies
Product description	Mouse monoclonal antibody [HIS.H8] to 6X His tagThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Immunogen	HHHHHH (6x His) synthetic peptide conjugated to KLH

Key Feature

Clonality	Monoclonal
Isotype	IgG2
Clone Number	HIS.H8
Host Species	Mouse
Tested Applications	ELISAICC/IFIPWB
	Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:3000. For ICC/IF: Use at a dilution of 1:100-1:500. For IP: Use at a dilution of 1:50-1:200. Optimal dilutions should be determined experimentally by the end user.:
Concentration	1 mg/ml
Purification	Affinity purified

Target Information

Gene Synonyms	6 His His tag 6 His epitope tag HHHHHH tag HHHHHH epitope tag
Target	6X His tag
Tissue Specificity	Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

Application

Application Notes	Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:3000. For ICC/IF: Use at a dilution of 1:100-1:500. For IP: Use at a dilution of 1:50-1:200. Optimal dilutions should be determined experimentally by the end user.:

Additional Information

Form	Liquid
Storage Instructions	Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage Buffer	10mM PBS, pH 7.2, 0.05% NaN3

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• Data sheet for OM237680