Mouse monoclonal [SPM368] to 6X His tagThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Immunogen
His tagged recombinant protein.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
SPM368
Host Species
Mouse
Tested Applications
ICC/IFWB
AP: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.:
Concentration
0.2 mg/ml
Purification
Protein A
Target Information
Gene Synonyms
6 His His tag 6 His epitope tag HHHHHH tag HHHHHH epitope tag 6X His tag
Target
6X His tag
Cellular Localization
Depends upon the localization of the parent protein tagged with hexa histidine.
Light Chain Type
kappa
Application
Positive Control
Hexa histidine fusion protein.
Application Notes
AP: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.:
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