6X His tag antibody

Product Profile

Product Name	6X His tag antibody
Antibody Type	Tags Antibodies
Product description	Mouse monoclonal [3H2201] to 6X His tagGTX15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
Immunogen	PAX6 transcription factor linked to 6-histidine tag

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	3H2201
Host Species	Mouse
Tested Applications	ELISAIPWB
	For ELISA: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the end user:
Concentration	0.5 mg/ml
Purification	Affinity purified

Target Information

Target	6X His tag
Tissue Specificity	Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. In Western Blot of bacterial extracts, the antibody does not crossreact with any endogenous products.

Application

Application Notes	For ELISA: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the end user:

Additional Information

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer	PBS, pH7.2 containing 0.1% sodium azide and 40% glycerol

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• Data sheet for OM237670