All products Antibodies 6X His tag antibody

6X His tag antibody

Catalog Number：OM237678

  • 6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] diluted at 1:2000. Blue: Hoechst 33342 staining.

  • 6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.

  • 6X His tag antibody [GT359] detects 6xHis Tag protein by western blot analysis. A. 2 µg GST-recombinant protein B. 2 µg His-recombinant protein C. 1 µg His-recombinant protein D. 0.5 µg His-recombinant protein E. 0.1 µg His-recombinant protein F. 0.05 µg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:3000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • 6X His tag antibody [GT359] detects 6x His protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • 6X His tag antibody [GT359] detects 6x His protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • 6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] diluted at 1:1000.

Product Profile

Product Name 6X His tag antibody
Antibody Type Tags Antibodies
Product description 
Mouse Monoclonal antibody [GT359] to 6X His tag

Key Feature

Clonality Monoclonal
Isotype IgG3
Clone Number GT359
Host Species Mouse
Tested Applications

ELISAICC/IFIPWB
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
6X His tag antibody
His antibody
6x His antibody
6x his tag antibody
His Tag antibody
6xHis Tag antibody
6 x His antibody

Application

  • Application

    6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] diluted at 1:2000. Blue: Hoechst 33342 staining.

  • Application

    6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.

  • Application

    6X His tag antibody [GT359] detects 6xHis Tag protein by western blot analysis. A. 2 µg GST-recombinant protein B. 2 µg His-recombinant protein C. 1 µg His-recombinant protein D. 0.5 µg His-recombinant protein E. 0.1 µg His-recombinant protein F. 0.05 µg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:3000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • Application

    6X His tag antibody [GT359] detects 6x His protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • Application

    6X His tag antibody [GT359] detects 6x His protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • Application

    6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] diluted at 1:1000.

Positive Control His-recombinant protein , IPTG-induced cell lysate from E. coli expressing His-tagged proteins
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer PBS
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM237678
  • Price: $396.00
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