AFP

Catalog Number：OM638930

  • Fig1: Western blot analysis of AFP on different lysates using anti-AFP antibody at 1/1,000 dilution. Postive control: Lane 1: MCF-7 Lane 2: PC-12 Lane 3: Human liver tissue

  • Fig2: ICC staining AFP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining AFP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining AFP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Fig8: Flow cytometric analysis of 239T cells with AFP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name AFP
Antibody Type Primary Antibodies
Product description 
α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.
Immunogen 
Recombinant protein.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:100
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
Afp antibody AFPD antibody Alpha fetoglobulin antibody Alpha fetoprotein antibody Alpha fetoprotein precursor antibody Alpha-1-fetoprotein antibody Alpha-fetoglobulin antibody Alpha-fetoprotein antibody alpha-fetoprotein
Hereditary persistence of
included antibody FETA antibody FETA_HUMAN antibody Hereditary persistence of alpha fetoprotein antibody HPAFP antibody
Cellular Localization Secreted.

Database Links

SwissProt ID P02771
P02772
P02773

Application

  • Application

    Fig1: Western blot analysis of AFP on different lysates using anti-AFP antibody at 1/1,000 dilution. Postive control: Lane 1: MCF-7 Lane 2: PC-12 Lane 3: Human liver tissue

  • Application

    Fig2: ICC staining AFP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining AFP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining AFP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AFP antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of 239T cells with AFP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control MCF-7, PC-12 cell lysate, human liver tissue lysate, HepG2, Hela, human liver cancer tissue, human breast cancer tissue, mouse liver tissue, 293T.
Application Notes
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:100
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638930
  • Price: $336.00
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