ASS1

Catalog Number:OM638944

  • Fig1: Western blot analysis of ASS1 on Hela (1) and MCF-7 (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.

  • Fig2: ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded 2 liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Fig8: Flow cytometric analysis of Jurkat cells with ASS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name ASS1
Antibody Type Primary Antibodies
Product description
ASS1, also known as argininosuccinate synthase or citrulline-aspartate ligase, belongs to the argininosuccinate synthase family. ASS1 is an urea cycle enzyme with a tetrameric structure composed of identical subunits. It is important to the urea cycle as it catalyzes the important second last step in the arginine biosynthetic pathway. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation.
Immunogen
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC

WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:200
Species Reactivity

HumanMouseRat

Concentration 1 mg/mL.

Target Information

Alternative Names
Argininosuccinate synthase 1 antibody Argininosuccinate synthase antibody Argininosuccinate synthetase 1 antibody ASS antibody Ass-1 antibody ass1 antibody ASSA antibody ASSY_HUMAN antibody Citrulline aspartate ligase antibody Citrullineaspartate ligase antibody CTLN1 antibody
Molecular Weight(MW)

47 kDa

Cellular Localization Cytoplasm.

Database Links

SwissProt ID P00966
P16460
P09034

Application

  • Application

    Fig1: Western blot analysis of ASS1 on Hela (1) and MCF-7 (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded 2 liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Flow cytometric analysis of Jurkat cells with ASS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, MCF-7, A549, NIH-3T3, Jurkat, human liver tissue, human kidney tissue, 2 liver tissue, 2 kidney tissue.
Application Notes
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM638944
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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