All products Antibodies Actin Antibody (H-196) FITC

Actin Antibody (H-196) FITC

Catalog Number:OM153677

  • Western blot analysis of Actin expression in A-10 whole cell lysate.

Product Profile

Product Name Actin Antibody (H-196) FITC
Antibody Type Primary Antibodies
Modification Notes All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. å-Actin expression is limited to various types of muscle, whereas -∫ and ©-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFFCELISA

WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope corresponding to amino acids 180–375 mapping at the C-terminus of Actin of human origin
Tissue Specificity epitope corresponding to amino acids 180–375 mapping at the C-terminus of Actin of human origin

Database Links

Entrez Gene 58

Application

  • Application

    Western blot analysis of Actin expression in A-10 whole cell lysate.

Application Notes
WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM153677
  • Price: $336.00
  • Discount Price
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  • Lead time: 4~6 Week
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