After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.
Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Goat
Tested Applications
ChIPFCIFIHCIPWB
WB:1:5000~1:10000
Species Reactivity
Rabbit
Concentration
1 mg/ml
Purification
Affinity purified
Application
Application
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.
Application Notes
WB:1:5000~1:10000
Additional Information
Form
Liquid
Storage Instructions
Storage:
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
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