Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).
Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).
Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Alligator
Tested Applications
ChIPFCIFIHCIPWB
Species Reactivity
Rat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.
Application
Application
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).
Application
Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).
Additional Information
Storage Instructions
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:500 - 1:2000Flow Cytometry: 1:500 - 1:1000
Storage:
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
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