All products Antibodies B7-H4(VTCN1)

B7-H4(VTCN1)

Catalog Number：OM638915

  • Fig1: Western blot analysis of B7-H4 on different lysate using anti-B7-H4 antibody at 1/1,000 dilution. Positive control：Lane1: SK-Br-3Lane2: Human liverLane3: Mouse testisLane4: Mouse spleen

  • Fig2: ICC staining B7-H4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining B7-H4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining B7-H4 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- B7-H4 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-B7-H4 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of Jurkat cells with B7-H4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Product Profile

Product Name B7-H4(VTCN1)
Antibody Type Primary Antibodies
Product description 
T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4, also designated VTCN1), belongs to the B7 Immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells supresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
B7 family member
H4 antibody B7 H4 antibody B7 homolog 4 antibody B7 superfamily member 1 antibody B7 superfamily
member 1 antibody B7-H4 antibody B7h.5 antibody B7h4 antibody B7S1 antibody B7x antibody BC032925 antibody Immune costimulatory protein B7-H4 antibody Immune costimulatory protein B7H4 antibody MGC41287 antibody PRO1291 antibody Protein B7S1 antibody RP11 229A19.4 antibody T cell costimulatory molecule B7x antibody T-cell costimulatory molecule B7x antibody V set domain-containing T cell activation inhibitor 1 antibody V-set domain-containing T-cell activation inhibitor 1 antibody VCTN1 antibody Vtcn1 antibody VTCN1_HUMAN antibody
Molecular Weight(MW) 38 kDa
Cellular Localization Cell membrane. Expressed at the cell surface.

Database Links

SwissProt ID Q7Z7D3
Q7TSP5

Application

  • Application

    Fig1: Western blot analysis of B7-H4 on different lysate using anti-B7-H4 antibody at 1/1,000 dilution. Positive control：Lane1: SK-Br-3Lane2: Human liverLane3: Mouse testisLane4: Mouse spleen

  • Application

    Fig2: ICC staining B7-H4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining B7-H4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining B7-H4 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- B7-H4 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-B7-H4 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of Jurkat cells with B7-H4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control SK-Br-3  cell lysate, human liver tissue lysate, mouse testis tissue lysate, mouse spleen tissue lysate, Hela, MCF-7, human tonsil tissue, human lung cancer tissue, Jurkat.
Application Notes
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638915
  • Price: $336.00
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