BECN1

Catalog Number：OM638883

  • Fig1： Immunocytochemical staining of N2A cells using anti-BECN1 rabbit polyclonal antibody.

  • Fig2： Immunocytochemical staining of Hela cells using anti-BECN1 rabbit polyclonal antibody.

  • Fig3： Immunocytochemical staining of MCF-7 cells using anti-BECN1 rabbit polyclonal antibody.

  • Fig4： Immunocytochemical staining of SHG-44 cells using anti-BECN1 rabbit polyclonal antibody.

  • Fig5： Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig6： Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig7： Immunohistochemical analysis of paraffin- embedded human breast tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig8： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig9： Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig10： Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-BECN1 rabbit polyclonal antibody.

  • Fig11： Flow cytometric analysis of HepG2 cells with BECN1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Product Profile

Product Name BECN1
Antibody Type Primary Antibodies
Product description 
BECN1 (Beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the Ribosomal Protein S6, and both are mediated via amino acids. BECN1 expression in various carcinoma cell lines such as MCF7 is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, Apg6, and was one of the first mammalian proteins discovered to mediate autophagy.
Immunogen 
Synthetic peptide (KLH-coupled) within human BECN1 C terminal.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICCIHCFC
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
APG6 antibody ATG 6 antibody ATG6 antibody ATG6 autophagy related 6 homolog antibody Bcl-2-interacting protein beclin antibody Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody Beclin 1 autophagy related antibody Beclin-1 antibody Beclin1 antibody BECN 1 antibody Becn1 antibody BECN1_HUMAN antibody Coiled coil myosin like BCL2 interacting protein antibody Coiled-coil myosin-like BCL2-interacting protein antibody GT197 antibody Protein GT197 antibody VPS 30 antibody VPS30 antibody
Molecular Weight(MW) 52 kDa
Cellular Localization Cytoplasm, Endosome, Mitochondrion membrane Mitochondrion membrane, Endoplasmic reticulum membrane

Database Links

SwissProt ID Q14457
O88597

Application

  • Application

    Fig1： Immunocytochemical staining of N2A cells using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig2： Immunocytochemical staining of Hela cells using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig3： Immunocytochemical staining of MCF-7 cells using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig4： Immunocytochemical staining of SHG-44 cells using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig5： Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig6： Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig7： Immunohistochemical analysis of paraffin- embedded human breast tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig8： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig9： Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig10： Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-BECN1 rabbit polyclonal antibody.

  • Application

    Fig11： Flow cytometric analysis of HepG2 cells with BECN1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Positive Control Hela, N2A, MCF-7, SHG-44, human liver cancer tissue, human breast tissue, human lung tissue, human kidney tissue, mouse brain tissue, mouse heart tissue, HepG2.
Application Notes
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638883
  • Price: $336.00
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  • Lead time: 4~6 Week

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