Fig1: Immunocytochemical staining of HepG2 cells using anti-Bad rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-Bad rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of SW480 cells using anti-Bad rabbit polyclonal antibody.
Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Bad rabbit polyclonal antibody.
Fig5: Flow cytometric analysis of MCF-7 cells with Bad antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes thereby causing restoration of Bax-mediated apoptosis.
Immunogen
Synthetic peptide within human Bad N-terminal (KLH-coupled).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBICCIHCFC
ICC:1:50-1:200 IHC:1:50-1:200
Species Reactivity
Human
Concentration
1 mg/mL.
Target Information
Alternative Names
AI325008 antibody
BAD antibody
BAD_HUMAN antibody
BBC 2 antibody
BBC2 antibody
BBC6 antibody
Bcl 2 Antagonist of Cell Death antibody
Bcl 2 Binding Component 6 antibody
BCL X / BCL 2 Binding Protein antibody
BCL X Binding Protein antibody
Bcl XL/Bcl 2 Associated Death Promoter antibody
Bcl-2-binding component 6 antibody
Bcl-2-like protein 8 antibody
Bcl-XL/Bcl-2-associated death promoter antibody
Bcl2 antagonist of cell death antibody
BCL2 antagonist of cell death protein antibody
BCL2 associated agonist of cell death antibody
Bcl2 Associated Death Promoter antibody
BCL2 binding component 6 antibody
BCL2 binding protein antibody
Bcl2 Like 8 Protein antibody
Bcl2-L-8 antibody
BCL2L8 antibody
Proapoptotic BH3 Only Protein antibody
Fig1: Immunocytochemical staining of HepG2 cells using anti-Bad rabbit polyclonal antibody.
Application
Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-Bad rabbit polyclonal antibody.
Application
Fig3: Immunocytochemical staining of SW480 cells using anti-Bad rabbit polyclonal antibody.
Application
Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Bad rabbit polyclonal antibody.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with Bad antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Positive Control
HepG2, SK-BR-3, SW480, human breast carcinoma tissue, MCF-7
Application Notes
ICC:1:50-1:200 IHC:1:50-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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