Fig1: Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution. Positive control:
Lane 1: Human brain
Lane2: Mouse brain
Lane3: Mouse testis
Lane 4: Mouse thymus
Lane5: Mouse spleen
Lane6:Jurkat
Lane7: TF-1
Lane8: F9
Fig2: ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- CD34 rabbit polyclonal antibody.
CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC
Fig1: Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution. Positive control:
Lane 1: Human brain
Lane2: Mouse brain
Lane3: Mouse testis
Lane 4: Mouse thymus
Lane5: Mouse spleen
Lane6:Jurkat
Lane7: TF-1
Lane8: F9
Application
Fig2: ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- CD34 rabbit polyclonal antibody.
Positive Control
Human brain, mouse brain, mouse testis, Mouse thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue
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