Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120.
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250.
Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
Application
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Positive Control
Mouse brain tissue, LOVO, Raji.
Application Notes
WB:1:500 ICC
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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