COX IV

Catalog Number：OM638949

  • Fig1: Western blot analysis of COX IV on rat heart tissue lysate using anti-COX IV antibody at 1/2,000 dilution.

  • Fig2: ICC staining COX IV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining COX IV in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Fig9: Flow cytometric analysis of Hela cells with COX IV antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name COX IV
Antibody Type Primary Antibodies
Product description 
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.
Immunogen 
Peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
AL024441 antibody COX 4 antibody COX IV 1 antibody COX IV antibody COX IV-1 antibody Cox4 antibody COX41_HUMAN antibody Cox4a antibody COX4B antibody COX4I1 antibody COX4I2 antibody COX4L2 antibody COXIV antibody Cytochrome c oxidase polypeptide IV antibody Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody Cytochrome c oxidase subunit 4 isoform 1
mitochondrial antibody Cytochrome C Oxidase subunit IV antibody Cytochrome c oxidase subunit IV isoform 1 antibody Cytochrome c oxidase subunit IV isoform 2 (lung) antibody Cytochrome c oxydase subunit 4 antibody dJ857M17.2 antibody MGC105470 antibody MGC72016 antibody
Molecular Weight(MW) 16 kDa
Cellular Localization Mitochondrion inner membrane.

Database Links

SwissProt ID P13073
P19783
P10888

Application

  • Application

    Fig1: Western blot analysis of COX IV on rat heart tissue lysate using anti-COX IV antibody at 1/2,000 dilution.

  • Application

    Fig2: ICC staining COX IV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining COX IV in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-COX IV antibody. Counter stained with hematoxylin.

  • Application

    Fig9: Flow cytometric analysis of Hela cells with COX IV antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Rat heart tissue, Hela, MCF-7, HepG2, human colon cancer tissue, human kidney tissue, mouse heart tissue.
Application Notes
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638949
  • Price: $336.00
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