Cyclin D1

Catalog Number：OM626569

  • Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

Product Profile

Product Name Cyclin D1
Antibody Type Primary Antibodies
Product description 
Cyclin D1 belongs to the family of cyclin proteins which function as the regulatory subunits of cyclin/cyclin dependent kinase holoenzymes. The cyclin D1 proto-oncogene is an important regulator of G1 to S-phase transition and an important cofactor for several transcription factors in numerous cell types. Cyclin D1 is known to modulate local chromatin structure and transcription of genes involved in proliferation and differentiation through CDK-independent association with histone acetylases and deacetylases.
Immunogen 
peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB 1:5,000:
ICC 1:200:
FC 1:50-1:100:
Species Reactivity

HumanMouseRatZebra Fish
Concentration 1 mg/mL.

Target Information

Alternative Names
AI327039 antibody B cell CLL/lymphoma 1 antibody B cell leukemia 1 antibody B cell lymphoma 1 protein antibody B-cell lymphoma 1 protein antibody BCL 1 antibody BCL-1 antibody BCL-1 oncogene antibody BCL1 antibody BCL1 oncogene antibody ccnd1 antibody CCND1/FSTL3 fusion gene
included antibody CCND1/IGHG1 fusion gene
included antibody CCND1/IGLC1 fusion gene
included antibody CCND1/PTH fusion gene
included antibody CCND1_HUMAN antibody cD1 antibody Cyl 1 antibody D11S287E antibody G1/S specific cyclin D1 antibody G1/S-specific cyclin-D1 antibody Parathyroid adenomatosis 1 antibody PRAD1 antibody PRAD1 oncogene antibody U21B31 antibody
Molecular Weight(MW) 34 kDa
Cellular Localization Cytoplasm, Membrane, Nucleus.

Database Links

SwissProt ID P24385
P25322
P39948

Application

  • Application

    Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Application

    Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • Application

    Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

  • Application

    Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

Positive Control MCF-7, A549, HepG2, N2A.
Application Notes
WB 1:5,000:
ICC 1:200:
FC 1:50-1:100:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626569
  • Price: $210.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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