Western blot analysis of (1) 20 ng of DDDDK-tagged recombinant GGP1 protein and (2) 20 ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Western blot analysis of (1) 1000ng, (2) 500 ng, (3) 250 ng, and (4) 125 ng of a DDDDK-tag-containing recombinant GGP1 protein with DDDDK-tag antibody at 0.5 μg/ml.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.
Immunogen
DDDDK-tag antibody was raised against a synthetic peptide containing DYKDDDDK.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Mouse
Tested Applications
ELISAIPWB
DDDDK-tag antibody can be used for detection of DDDDK-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Western blot analysis of (1) 20 ng of DDDDK-tagged recombinant GGP1 protein and (2) 20 ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Application
Western blot analysis of (1) 1000ng, (2) 500 ng, (3) 250 ng, and (4) 125 ng of a DDDDK-tag-containing recombinant GGP1 protein with DDDDK-tag antibody at 0.5 μg/ml.
Positive Control
1) N/A
Application Notes
DDDDK-tag antibody can be used for detection of DDDDK-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Additional Information
Form
Liquid
Storage Instructions
DDDDK-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer
DDDDK-tag antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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