FGF-Basic Thermostable is a stabilized form of FGF-2 growth factor which enables a novel method to produce FGF2-dependent cell cultures more efficiently, having less media changes. FGF-Basic Thermostable can maintain its biological activity even after five days at 37°C. The increase in the stability of FGF2 in cell-culture enables a more homogenous, undifferentiated stem cell culture, while saving scientists crucial time and money, as frequent supplementation of FGF-basic and the everyday medium change is not necessary. Thermostable FGF-2 is a hyperstable protein. The Thermal stability of the protein is increased by 15°C compared to the wild-type FGF-2. Thermostable FGF-2 is more than 5-times prolonged half-life in human cell culture incubated at 37°C. The FGF-2 Thermostable protein is engineered with fully retained biological function and has no harmful stabilizing additives.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Storage Buffer
FGF2 was filtered and lyophilized from a solution containing 20mM potassium phosphate buffer and 750mM sodium chloride, pH 7.5.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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