GFP antibody

GFP antibody detects GFP protein by western blot analysis. A. 5 µg 293T whole cell extract B. 5 µg 293T whole cell extract expressing GFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ELISA detection of GFP using for capture and for detection. Coating Ab: mouse anti-GFP (, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP ( 1:20000)

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody diluted at 1:200. Blue: Hoechst 33342 staining.