All products Antibodies GFP antibody

GFP antibody

Catalog Number：OM254472

  • 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used

Product Profile

Product Name GFP antibody
Antibody Type Tags Antibodies
Product description 
Mouse monoclonal antibody [GF28R] to GFPA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.
Immunogen 
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number GF28R
Host Species Mouse
Tested Applications

ELISAICC/IFIPWB
WB (with ECL): Use at a dilution of 1:1000-3000 :
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
eGFP
Green Fluorescent Protein
GFP
GFP Tag
Enhanced Green Fluorescent Protein
Target GFP
Tissue Specificity Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Application

  • Application

    1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used

Application Notes
WB (with ECL): Use at a dilution of 1:1000-3000 :

Additional Information

Form Liquid
Storage Instructions Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles
Storage Buffer 10mM PBS, pH 7.2
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM254472
  • Price: $396.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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