All products Antibodies GFP antibody

GFP antibody

Catalog Number：OM254473

  • ELISA detection of GFP using for capture and for detection. Coating Ab: mouse anti-GFP (, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP ( 1:20000)

  • GFP antibody detects GFP protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

Product Profile

Product Name GFP antibody
Antibody Type Tags Antibodies
Product description 
Mouse Monoclonal antibody to GFP (Green Fluorescent Protein)

Key Feature

Clonality Monoclonal
Isotype IgG2a
Clone Number GT7312
Host Species Mouse
Tested Applications

ELISAIPWB
*Optimal dilutions/concentrations should be determined by the researcher. :
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Molecular Weight(MW) 29 kDa (note)
Tissue Specificity This antibody reacts with GFP variants including GFP, EGFP, and YFP.

Application

  • Application

    ELISA detection of GFP using for capture and for detection. Coating Ab: mouse anti-GFP (, 2µg/well ) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP ( 1:20000)

  • Application

    GFP antibody detects GFP protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12% SDS-PAGE GFP antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

  • Application

    GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

Positive Control 293T whole cell extract expressing GFP-tagged protein
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer PBS
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM254473
  • Price: $396.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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