All products Antibodies GM130 (cis-Golgi Marker)

GM130 (cis-Golgi Marker)

Catalog Number：OM638946

  • Fig1: Western blot analysis of GM130 on different cell lysate using anti-GM130 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: Hela

  • Fig2: ICC staining GM130 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of Hela cells with GM130 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name GM130 (cis-Golgi Marker)
Antibody Type Primary Antibodies
Product description 
Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly. Also plays a key role in spindle pole assembly and centrosome organization. Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle. Regulates the meiotic spindle pole assembly, probably via the same mechanism. Also regulates the centrosome organization. Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:200
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
30 kDa cis Golgi matrix protein antibody 130 kDa cis-Golgi matrix protein antibody Cis golgi matrix protein GM130 antibody GM130 antibody Gm130 autoantigen antibody GOGA2_HUMAN antibody GOLGA 2 antibody Golga2 antibody Golgi autoantigen antibody Golgi autoantigen golgin subfamily a 2 antibody Golgi matrix protein GM130 antibody Golgin 95 antibody golgin A2 antibody Golgin subfamily a 2 antibody Golgin subfamily A member 2 antibody Golgin-95 antibody MGC20672 antibody SY11 protein antibody
Molecular Weight(MW) 130 kDa
Cellular Localization Cytoplasm, Cytoskeleton, Membrane, Microtubule.

Database Links

SwissProt ID Q08379
Q921M4
Q62839

Application

  • Application

    Fig1: Western blot analysis of GM130 on different cell lysate using anti-GM130 antibody at 1/1,000 dilution. Positive control： Lane1: MCF-7 Lane2: Hela

  • Application

    Fig2: ICC staining GM130 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GM130 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of Hela cells with GM130 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, MCF-7, LO2, human tonsil tissue, human liver tissue, human kidney tissue, mouse brain tissue.
Application Notes
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638946
  • Price: $336.00
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