GPC1

Catalog Number：OM638922

  • Fig1: Western blot analysis of Glypican-1 on different cell lysate using anti- Glypican-1 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: SK-Br-3

  • Fig2: ICC staining Glypican-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Glypican-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining Glypican-1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of MCF-7 cells with Glypican-1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name GPC1
Antibody Type Primary Antibodies
Product description 
Glypican-1 (GPC1), a member of the glycosylphosphatidilinositol anchored cell surface heparan sulfate proteoglycans, is involved with cell adhesion and migration, lipoprotein metabolism, modulation of growth factor activites and anticoagulation. Glypican-1 binds to and modulates the activity of several fibroblast growth factors (FGFs) including FGF-1, FGF-2 and FGF-7. Glypican-1 acts as an extracellular chaperone for VEGF165 to help restore receptor binding ability after oxidation. The heparan sulfate chains of glypican-1 mediate specific binding of glypican-1 to VEGF165. When present on the surface of marrow stromal cells, glypican-1 may aid in the maintenance and development of hematopoietic stem and progenitor cells. Human pancreatic cancer cells express a large amount of glypican-1 when compared to glypican-1 levels in normal pancreatic cells. Glypican-1 may play an important role in the response of pancreatic cancer cells to mitogenic stimuli, such as FGF-2. The gene encoding human glypican-1 maps to chromosome 2q37.3.
Immunogen 
Peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

Human
Concentration 1 mg/mL.

Target Information

Alternative Names
FLJ38078 antibody glypican antibody Glypican proteoglycan 1 antibody glypican1 antibody GPC 1 antibody Gpc1 antibody GPC1_HUMAN antibody Secreted glypican-1 antibody
Molecular Weight(MW) 61 kDa
Cellular Localization Membrane. Secreted.

Database Links

SwissProt ID P35052

Application

  • Application

    Fig1: Western blot analysis of Glypican-1 on different cell lysate using anti- Glypican-1 antibody at 1/1,000 dilution. Positive control： Lane1: Hela Lane2: SK-Br-3

  • Application

    Fig2: ICC staining Glypican-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Glypican-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining Glypican-1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of MCF-7 cells with Glypican-1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, SK-Br-3, MCF-7, PANC-1, human breast tissue, human breast cancer tissue.
Application Notes
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638922
  • Price: $336.00
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