All products Antibodies GST Antibody

GST Antibody

Catalog Number：OM280432

  • Western blot analysis using GST monoclonal antibody against GST recombinant protein.

Product Profile

Product Name GST Antibody
Antibody Type Tags Antibodies
Product description 
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.1) Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.
Immunogen 
Ni-NTA purified recombinant GST expressed in E. Coli strain M15.

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

ELISAWB
Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.:
Species Reactivity

GST
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol gst
Alternative Names
gst
b1635
JW1627
GST B1-1

Database Links

Entrez Gene 12931264
Protein Accession P0A9D2

Application

  • Application

    Western blot analysis using GST monoclonal antibody against GST recombinant protein.

Application Notes
Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.:

Additional Information

Form Liquid
Storage Instructions GST monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Purified antibody in PBS containing 0.03% sodium azide
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM280432
  • Price: $396.00
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  • Lead time: 4~6 Week

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