Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.1) Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.
Immunogen
Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAWB
Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.:
Western blot analysis using GST monoclonal antibody against GST recombinant protein.
Application Notes
Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
GST monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Purified antibody in PBS containing 0.03% sodium azide
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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