Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBIPIFFCELISA
WB, IP, IF, FCM and ELISA of recombinant GST fusion proteins expressed in E. coli; designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Alternative Names
recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins
Tissue Specificity
recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins
Application
Application
Western blot analysis of human recombinant NFκB p50 (A) and GST (B) fusion proteins.
Application Notes
WB, IP, IF, FCM and ELISA of recombinant GST fusion proteins expressed in E. coli; designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
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