Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng Lane 2: 50ng Lane 3: 25ng Lane 4: 10ng Lane 5: 5ng Lane 6: 2ng Lane 7: 1ng
ELISA: Use at a concentration of 0.05 μg. IP: Use at a concentration of 1 - 10 μg. WB: Use at 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRPO/ECL detection.Predicted molecular weight: 25kDa. Optimal dilutions/concentrations should be determined by the end user.:
Concentration
1 mg/ml
Purification
Protein G
Target Information
Gene Synonyms
GST
Target
GST
Tissue Specificity
Specifically interacts with GST of Schistosoma japonicum that is encoded by the pGEX expression vectors.
Cellular Localization
Cytoplasmic and Nuclear
Light Chain Type
kappa
Application
Application
Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng Lane 2: 50ng Lane 3: 25ng Lane 4: 10ng Lane 5: 5ng Lane 6: 2ng Lane 7: 1ng
Application Notes
ELISA: Use at a concentration of 0.05 μg. IP: Use at a concentration of 1 - 10 μg. WB: Use at 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRPO/ECL detection.Predicted molecular weight: 25kDa. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
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