All products Antibodies GST antibody

GST antibody

Catalog Number：OM255672

  • Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02)

Product Profile

Product Name GST antibody
Antibody Type Tags Antibodies
Product description 
Mouse Monoclonal antibody [S-tag-05] to GSTThe GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Immunogen 
GST fusion protein

Key Feature

Clonality Monoclonal
Isotype IgG2
Clone Number S-tag-05
Host Species Mouse
Tested Applications

IPWB
WB: Use at 1 µg/mL. Positive Control: GST fusion protein. Note: Reducing conditions:
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
Glutathione-S-Transferase
GST Tag
Target GST
Tissue Specificity The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum . This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli , yeast, mammalian, and in vitro transcription/translation systems.

Application

  • Application

    Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.Western blot was immunostained by anti-GST (S-tag-05). Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose. Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02)

Application Notes
WB: Use at 1 µg/mL. Positive Control: GST fusion protein. Note: Reducing conditions:

Additional Information

Form Liquid
Storage Instructions Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.
Storage Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM255672
  • Price: $396.00
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  • Lead time: 4~6 Week

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