All products Antibodies Goat Anti-Mouse IgG H&L (TRITC)

Goat Anti-Mouse IgG H&L (TRITC)

Catalog Number：OM626549

  • Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

  • Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.

  • Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.

Product Profile

Product Name Goat Anti-Mouse IgG H&L (TRITC)
Antibody Type Secondary Antibodies
Product description 
This product is goat anti-mouse IgG secondary antibody labeled NHS-Rhodamine, can be used in ICC.
Immunogen 
Mouse IgG1

Key Feature

Clonality Polyclonal
Isotype IgG1
Host Species Goat
Tested Applications

ICC/IFFC
ICC/IF:1:50-1:200
FC:1:50-1:200
Species Reactivity

Mouse
Concentration 2 mg/mL.

Application

  • Application

    Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

  • Application

    Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.

  • Application

    Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.

Application Notes
ICC/IF:1:50-1:200
FC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626549
  • Price: $210.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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