Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.
Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.
Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.
Product Name | Goat Anti-Mouse IgG H&L (TRITC) |
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Antibody Type | Secondary Antibodies |
Product description |
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Immunogen | Mouse IgG1 |
Clonality | Polyclonal |
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Isotype | IgG1 |
Host Species | Goat |
Tested Applications | |
ICC/IF:1:50-1:200 FC:1:50-1:200 |
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Species Reactivity | |
Concentration | 2 mg/mL. |
Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.
Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.
Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.
Application Notes | ICC/IF:1:50-1:200 FC:1:50-1:200 |
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Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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