All products Antibodies Goat Anti-Rabbit IgG H&L (TRITC)

Goat Anti-Rabbit IgG H&L (TRITC)

Catalog Number：OM626545

  • Fig1: ICC staining Fascin(ET1705-18)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

  • Fig2: ICC staining SMC3(ET1703-35)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.

  • Fig3: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (ET1606-11) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (TRITC) (HA1016) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/200 dilution.

  • Fig4: Flow cytometric analysis of Hela cells with SMC3 (ET1703-35) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/100 dilution.

Product Profile

Product Name Goat Anti-Rabbit IgG H&L (TRITC)
Antibody Type Secondary Antibodies
Product description 
This product is goat anti-rabbit IgG secondary antibody labeled NHS-Rhodamine, can be used in ICC and FC.
Immunogen 
Rabbit IgG

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFFC
ICC/IF:1:50-1:200
FC:1:50-1:100:
Species Reactivity

Rabbit
Concentration 1 mg/mL.

Application

  • Application

    Fig1: ICC staining Fascin(ET1705-18)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

  • Application

    Fig2: ICC staining SMC3(ET1703-35)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.

  • Application

    Fig3: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (ET1606-11) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (TRITC) (HA1016) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/200 dilution.

  • Application

    Fig4: Flow cytometric analysis of Hela cells with SMC3 (ET1703-35) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/100 dilution.

Application Notes
ICC/IF:1:50-1:200
FC:1:50-1:100:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626545
  • Price: $210.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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