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HA Tag Antibody

Catalog Number：OM234703

  • Western blot analysis of anti-HA tag pabin HA-tagged recombinant protein bacterial lysate.

  • GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))

  • ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))

Product Profile

Product Name HA Tag Antibody
Antibody Type Tags Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIF
WB~~1:50~100 IF~~1:10~50 CHIP~~1:100:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Molecular Weight(MW) 25712 Da
Tissue Specificity KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

Application

  • Application

    Western blot analysis of anti-HA tag pabin HA-tagged recombinant protein bacterial lysate.

  • Application

    GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))

  • Application

    ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))

Application Notes
WB~~1:50~100 IF~~1:10~50 CHIP~~1:100:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM234703
  • Price: $336.00
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