Rabbit polyclonal to HA TagEpitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
Immunogen
Synthetic Peptide: YPYDVPDYA
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
For WB: Use at a dilution of 1:200. This antibody detects the presence of HA-tagged proteins. Optimal dilutions/concentrations should be determined by the end user.:
Concentration
0.2 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
HA epitope tag
Target
HA tag
Tissue Specificity
Detects HA tagged proteins
Application
Application Notes
For WB: Use at a dilution of 1:200. This antibody detects the presence of HA-tagged proteins. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
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