HA tag antibody

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 µg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

HA Tag antibody [GT423] detects HA Tag protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of HA-tagged protein expressing HeLa cells 7.5% SDS-PAGE HA Tag antibody [GT423] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

HA tag antibody [GT423] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA-recombinant protein stained by HA tag antibody [GT423] diluted at 1:2000. Blue: Hoechst 33342 staining.

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments. IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells A. Control with 2 μg of preimmune mouse IgG B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] 5% SDS-PAGE The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody diluted at 1:1000.