Rabbits were immunized with QPELAPEDPEDC conjugated with KLH.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAWB
Western blotting: use at a dilution of 1/500 - 1/1000. ELISA: use at a dilution of 1/5000 dilution. This gave an O.D.=1.0 in a 15 minute reaction using an HRP conjugated donkey anti rabbit (IgG) at 1/18000 and TMB as the substrate. Optimal dilutions / concentrations are best determined by the end user.:
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
HSV epitope tag
Target
HSV tag
Tissue Specificity
Antibody specificity was verified by direct ELISA against the peptide conjugated to BSA.
Application
Application Notes
Western blotting: use at a dilution of 1/500 - 1/1000. ELISA: use at a dilution of 1/5000 dilution. This gave an O.D.=1.0 in a 15 minute reaction using an HRP conjugated donkey anti rabbit (IgG) at 1/18000 and TMB as the substrate. Optimal dilutions / concentrations are best determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C.
Storage Buffer
PBS with 0.1% sodium azide, pH 7.4
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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