Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.
Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. His-tag is one of the more popular epitope tags for expressed recombinant proteins.
Immunogen
His-tag antibody was raised against a synthetic peptide containing HHHHHH.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIPWB
His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.
Application
Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Positive Control
1) N/A
Application Notes
His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.:
Additional Information
Form
Liquid
Storage Instructions
His-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer
His-tag antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.