IKK beta

Catalog Number：OM638952

  • Fig1: Western blot analysis of IKK beta on different lysates using anti-IKK beta antibody at 1/1,000 dilution. Positive control： Lane1: HelaLane2: 293TLane3: Mouse spleen tissue

  • Fig2: ICC staining IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining IKK beta in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Fig9: Flow cytometric analysis of A549 cells with IKK beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name IKK beta
Antibody Type Primary Antibodies
Product description 
The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates Iκβ-α on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
I kappa B kinase 2 antibody I kappa B kinase beta antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody IkBKB antibody IKK beta antibody IKK-B antibody IKK-beta antibody IKK2 antibody IKKB antibody IKKB_HUMAN antibody IMD15 antibody Inhibitor of kappa light polypeptide gene enhancer in B cells
kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKB antibody Nuclear factor NF-kappa-B inhibitor kinase beta antibody
Molecular Weight(MW) 87/79 kDa
Cellular Localization Nucleus. Cytoplasm.

Database Links

SwissProt ID O14920
O88351
Q9QY78

Application

  • Application

    Fig1: Western blot analysis of IKK beta on different lysates using anti-IKK beta antibody at 1/1,000 dilution. Positive control： Lane1: HelaLane2: 293TLane3: Mouse spleen tissue

  • Application

    Fig2: ICC staining IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining IKK beta in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Application

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IKK beta antibody. Counter stained with hematoxylin.

  • Application

    Fig9: Flow cytometric analysis of A549 cells with IKK beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, 293T, mouse spleen tissue lysate, HUVEC, rat testis tissue, human tonsil tissue, human breast tissue, human placenta tissue, mouse testis tissue.
Application Notes
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638952
  • Price: $336.00
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  • Lead time: 4~6 Week

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