Fig1: Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of Daudi cells with IL-7R-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Interleukin 7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor g chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2), originally called 4PS.
Fig1: Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin.
Application
Fig3: Flow cytometric analysis of Daudi cells with IL-7R-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Positive Control
Zebrafish, Daudi.
Application Notes
WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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