IL-8

Catalog Number：OM638888

  • Fig1: Western blot analysis of IL-8 on human kidney tissue lysate using anti-IL-8 antibody at 1/1,000 dilution.

  • Fig2: ICC staining IL-8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: Flow cytometric analysis of HUVEC cells with IL-8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Profile

Product Name IL-8
Antibody Type Primary Antibodies
Product description 
Interleukin-8, or IL-8, the prototypic member of the C-X-C, or a, family of chemokines, is a chemoattractant cytokine involved in the chemotaxis and activation of neutrophils. IL-8 expression has been correlated to a large number of chronic inflammatory diseases, including inflammatory bowel disease (IBD) and atherosclerosis. IL-8 is cleaved from a 99 amino acid precursor to a 72 amino acid, nonglycosylated, biologically active protein. IL-8 monomers and dimers exhibit a dynamic equilibrium both free in solution and in cell surface-bound forms and thus regulate chemotaxis and receptor signaling. Research has shown that IL-8 dimerization functions as a negative regulator for IL-8 receptor function. Two IL-8 receptors, designated IL-8RA and IL-8RB, have been described and share 77% sequence identity. Both are seven-transmembrane domain proteins (7TMD), similar to the G protein-coupled receptors and, in addition to IL-8, serve as receptors for other members of the a and b chemokine families.
Immunogen 
Recombinant full length protein of human IL-8.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCFC
WB:1:500-1:2,000
ICC:1:500-1:1,000
FC:1:50-1:100
Species Reactivity

Human
Concentration 1 mg/mL.

Target Information

Alternative Names
(Ala-IL-8)77 antibody (Ser-IL-8)72 antibody 9E3 antibody Beta thromboglobulin like protein antibody C-X-C motif chemokine 8 antibody CEF-4 antibody chemokine
CXC motif
ligand 8 antibody CXCL8 antibody Emoctakin antibody GCP-1 antibody GCP/IL-8 protein I antibody GCP/IL-8 protein II antibody GCP/IL-8 protein III antibody GCP/IL-8 protein IV antibody GCP/IL-8 protein V antibody GCP/IL-8 protein VI antibody GCP1 antibody Granulocyte chemotactic protein 1 antibody IL-8 antibody IL-8(1-77) antibody IL-8(9-77) antibody IL8 antibody IL8/NAP1 form I antibody IL8/NAP1 form II antibody IL8/NAP1 form III antibody IL8/NAP1 form IV antibody IL8/NAP1 form V antibody IL8/NAP1 form VI antibody IL8_HUMAN antibody Inteleukin 8 antibody LECT antibody LUCT antibody Lymphocyte-derived neutrophil-activating factor antibody LYNAP antibody MDNCF antibody MDNCF-b antibody MDNCF-c antibody MONAP antibody Monocyte-derived neutrophil chemotactic factor antibody Monocyte-derived neutrophil-activating peptide antibody NAF antibody NAP-1 antibody NAP1 antibody Neutrophil activating peptide 1 antibody Neutrophil activating protein 1 antibody Neutrophil-activating factor antibody Neutrophil-activating protein 1 antibody Protein 3 10C antibody Protein 3-10C antibody SCYB8 antibody Small inducible cytokine subfamily B member 8 antibody T cell chemotactic factor antibody T-cell chemotactic factor antibody TSG1 antibody
Molecular Weight(MW) 11 kDa
Cellular Localization Secreted.

Database Links

SwissProt ID P10145

Application

  • Application

    Fig1: Western blot analysis of IL-8 on human kidney tissue lysate using anti-IL-8 antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining IL-8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Flow cytometric analysis of HUVEC cells with IL-8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control Human kidney tissue, 293T, HUVEC.
Application Notes
WB:1:500-1:2,000
ICC:1:500-1:1,000
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638888
  • Price: $336.00
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