Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.
Western blot analysis of IL-8RB expression in whole cell lysates prepared from CHO-K1 cells transfected with IL-8RB (A) and non-transfected CHO-K1 cells (B).
Intracellular FCM analysis of fixed and permeabilized non-transfected (green line histogram) and IL-8RB transfected (solid orange histogram) CHO-K1 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Product Name | IL-8RB Antibody (K-19) |
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Antibody Type | Primary Antibodies |
Modification Notes | IL-8 has been shown to function as a potent neutrophil chemostatic and activating peptide and is an important mediator of inflammatory diseases. Two distinct human IL-8 receptors, designated IL-8RA and IL-8RB, have been characterized. Both are expressed at a high level on neutrophils, and to a lesser extent on monocytes and myeloid cell lines. In addition, the IL-8RA subunit is expressed in T cells such as the Jurkat cell line. Both IL-8Rs are members of the seven transmembrane domain rhodopsin superfamily of receptors and as such, couple G proteins for signal transduction. The two receptors share 77% amino acid identity. IL-8RA exhibits high affinity binding for IL-8 and low affinity MGSA binding, whereas IL-8RB has high affinity binding for both IL-8 and MGSA. |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
recommended for detection of IL-8RB of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, FCM and ELISA: |
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Species Reactivity | |
Concentration | 1mg/ml |
Purification | Affinity purified |
Alternative Names | epitope mapping at the C-terminus of IL-8RB of mouse origin |
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Tissue Specificity | epitope mapping at the C-terminus of IL-8RB of mouse origin |
Entrez Gene | 3579 |
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Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.
Western blot analysis of IL-8RB expression in whole cell lysates prepared from CHO-K1 cells transfected with IL-8RB (A) and non-transfected CHO-K1 cells (B).
Intracellular FCM analysis of fixed and permeabilized non-transfected (green line histogram) and IL-8RB transfected (solid orange histogram) CHO-K1 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .
Application Notes | recommended for detection of IL-8RB of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, FCM and ELISA: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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