Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
Immunogen
peptide
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Mouse
Tested Applications
WBICCIHCFCIP
WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 IP:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Fig1: Western blot analysis of Iba1 on human PBL whole cell lysate using anti-Iba1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat C6 glioblastoma tissue using anti-Iba1 antibody. Counter stained with hematoxylin.
Positive Control
Human PBL whole cell lysate, rat brain, rat C6 glioblastoma tissue.
Application Notes
WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 IP:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher.
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