Western blot analysis of LTA Antibody (Center) (Cat. #AP8509c) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | LTA Antibody (Center) |
---|---|
Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
---|---|
Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | TNFB TNFSF1 |
---|---|
Alternative Names | LTA TNFB TNFSF1 Lymphotoxin-alpha TNF-beta Tumor necrosis factor ligand superfamily member 1 |
Molecular Weight(MW) | 22297 Da |
Function | Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo |
Tissue Specificity | This LTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52~81 amino acids from the Center region of human LTA. |
Cellular Localization | Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated |
Entrez Gene | 4049 |
---|
Western blot analysis of LTA Antibody (Center) (Cat. #AP8509c) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
---|
Form | Liquid |
---|---|
Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
2013 © Omnimabs , All Rights Reserved.